抗生素菌渣及有机肥基料、作物、环境 介质中青霉素检测方法 Method for determination of penicillin in antibiotic fermentation residue, raw fertilizer material, crop, and related environments （发布稿） 2021 -08-01发布 2021 -08-01实施 中国化学制药工业协会 发 布PIAC 中国化学制药工业协会团体标准 T/PIAC 00001 —2021 全国团体标准信息平台 T/PIAC 00001 —2021 I 前 言 本标准按照 GB/T 1.1 -2020的规则起草。 本标准由中国家环境保护抗生素菌渣无害化处理与资源化利用工程技术中心提出 。 本标准由中国化学 制药工业协会 归口。 本文件起草单位： 国家环境保护抗生素菌渣无害化处理与资源化利用工程技术中心、 中国 科学院生态环境研究中心、同济大学、中国环境科学研究院、北京农业生物技术研究中心、 清华大学、新疆大学、哈尔滨工业大学、常州大学、中广核达胜科技有限公司。 本文件主要起草人 ：沈云鹏、任立人 、张昱、王建龙 、杨敏、刘惠玲 、田书磊、王旭明、 贾殿赠、潘广成、雷英、邓留杰、董丽萍、王鲁香、任建军、冯皓迪、韩子铭、戴晓虎、邓 葵、刘宏博、何仕均、刘尊奇、刘胜辉、周春华、张远斌、冯书娟、刘希禹、牛东泽、张幼 学、 殷文娟、刘淑云、陈启军、地尔夏提.艾沙江、李 志杰。 本标准首批承诺执行单位：国家环境保护抗生素菌渣无害化处理与资源化利用工程技术 中心、中国科学院生态环境研究中心 、同济大学 、中国环境科学研究院 、清华大学 、新疆大学、 北京农业生物技术研究中心 、常州大学 、新疆农业大学 、新疆环境科学研究院 、伊犁州环境监 测站、伊犁州检验检测认证研究院、伊犁川宁生物技术股份有限公司。 全国团体标准信息平台 T/PIAC 0000 1—2021 II 目 录 1 范围 ································ ································ ································ ·············· 1 2 规范性引用文件 ································ ································ ······························· 1 3 抗生素菌渣及有机肥基料中青霉素 G的测定 液相色谱法 ································ ········ 1 3.1 原理 ································ ································ ································ ······ 1 3.2 试剂和材料 ································ ································ ····························· 1 3.2.1 试剂 ································ ································ ····························· 1 3.2.2 试剂配制 ································ ································ ······················· 2 3.2.3 标准品 ································ ································ ·························· 2 3.2.4 标准溶液配制 ································ ································ ················· 2 3.2.5 材料 ································ ································ ····························· 2 3.3 仪器设备 ································ ································ ································ 2 3.4 样品 ································ ································ ································ ······ 3 3.5 分析步骤 ································ ································ ································ 3 3.5.1 提取 ································ ································ ····························· 3 3.5.2 净化 ································ ································ ····························· 3 3.5.3 测定 ································ ································ ····························· 3 3.5.4 加标实验 ································ ································ ······················· 4 3.5.5 平行试验 ································ ································ ······················· 4 3.6 结果计算 ································ ································ ································ 4 3.7 准确度和精密度 ································ ································ ······················· 4 4 土壤中青霉素 G的测定 液相色谱 -串联质谱法 ································ ······················ 5 4.1 原理 ································ ································ ································ ······ 5 4.2 试剂和材料 ································ ································ ····························· 5 4.2.1 试剂 ································ ································ ····························· 5 4.2.2 试剂配制 ································ ································ ······················· 5 4.2.3 标准品 ································ ································ ·························· 5 4.2.4 标准溶液配制 ································ ································ ················· 5 4.2.5 材料 ································ ································ ····························· 5 4.3 仪器设备 ································ ·······························