牛羊副流感病毒3型诊断技术 Diagnostic techniques for bovine and caprine parainfluenza virus type 3  ICS  11.220 CCS  B 41   中华人民共和国国家标准 GB/T 44613—2024 2024-09-2 9发布 2025-04-0 1实施     国家市场监督管理总局 国家标准化管理委员会 发 布 目　　次 前言   ·····································································································   Ⅲ 引言   ·····································································································   Ⅳ 1     范围   ··································································································   1 2     规范性引用文件   ······················································································   1 3     术语和定义   ···························································································   1 4     缩略语   ································································································   1 5     临床诊断   ······························································································   2 6     样品采集、处理与保存   ···············································································   2 7     病毒分离鉴定   ·························································································   4 8     RT﹘PCR试验   ·························································································   5 9     Real﹘time RT﹘PCR试验   ···············································································   6 10     cELISA试验   ·························································································   7 11     病毒中和试验   ·······················································································   9 12     综合判定   ···························································································   11 附录 A （规范性）　试剂配制   ··········································································   12 附录 B （规范性）　病毒核酸检测用引物和荧光探针序列   ············································   13 附录 C （规范性）　RT﹘PCR 和 Real﹘time RT﹘PCR 用阳性对照品制备、引物及序列   ················   14 附录 D （规范性）　RT﹘PCR 和 Real﹘time RT﹘PCR 反应体系及反应程序   ····························   16 附录 E （资料性）　RT﹘PCR和Real﹘time RT﹘PCR试验结果图   ······································   18 附录 F （资料性）　病毒标准阳性血清、标准阴性血清和抗原包被酶标板的制备   ····················   20 附录 G （资料性）　酶标单克隆抗体的制备   ···························································   21 GB/T 44613—2024     Ⅰ